Trichosporon asahii is a major causative agent of deepseated trichosporonosis, which has a high mortality rate. Phenol utilization by filamentous yeast trichosporon cutaneum article pdf available in bioprocess and biosystems engineering 204. The gene encoding this enzyme was sequenced using 3. There are a few reports of genetic transformation systems for t. Trichosporon spp an emerging fungal pathogen medicina. Comparison of growth kinetics and phenol metabolizing. This pdf is available for free download from a site hosted by medknow publications. Infection with trichosporon pullulans annals of internal. This reaction initiates a metabolic sequence permitting utilisation of the aromatic compound as a source of carbon and energy. The culture medium consists of 5% glycerol, 5% corn steep liquor, 1% mineral mixture and 1% 1 m potassium phosphate buffer, ph 7. Over the past decade, the taxonomy of the genus trichosporon has been subjected to extensive revision on the basis of molecular data, and the previously named t. In strains of the yeast species trichosporon cutaneum septal pores of the basidiomycetetype were observed by electron microscopy. Successful treatment of trichosporon mucoides infection with. Trichosporon species are ureasepositive, nonencapsulated basidiomycetous yeasts characterised by the development of hyaline, septate hyphae that fragment into oval or rectangular arthroconidia.
Nevertheless, in 1942 diddens and lodder considered t. Osao adachi, in handbook of proteolytic enzymes third edition, 20. Conventional methods of its detection are timeconsuming or costly and often require complex dna extraction and purification steps, which hinders rapid clinical diagnosis. Trichosporon inkin peritonitis treated with caspofungin. Identification of trichosporon asahii by pcr based on. Merz, successful treatment of trichosporon mucoides infection with fluconazole in a heart and kidney transplant recipient. Clinical isolates are generally related to superficial infections. Rapid and simple detection of trichosporon asahii by. Dimorphism of trichosporon cutaneum and impact on its. Feb 12, 2018 voriconazole and posaconazole show excellent in vitro activity against trichosporon.
Candida krusei dbm 26, trichosporon cutaneum ccy 30510, and yarrowia lipolytica ccy 302636. Approximately 50 species of the genus trichosporon have been characterized, 16 of which are associated with diseases in humans. Veenhuis 2 1laboratory of microbiology, agricultural university, wageningen, the netherlands. Trichosporon trikosporon, trikosporon, a genus of imperfect fungi that possess branching septate hyphae with arthroconidia and blastoconidia. The present paper shows that such phenolinduced cells contain a specific, energydependent uptake system for phenol. Phenol utilization by filamentous yeast trichosporon cutaneum. Race cloning of the tal cdna and the gene was expressed in the. Amplification products were selectively obtained from only t. Trichosporon cutaneum is shown to utilize six disaccharides, cellobiose, maltose, lactose, sucrose, melibiose, and trehalose. Molecular identification of trichosporon species pdf. Yeasts of the anascosporogenous genus trichosporon are characterized by the formation of budding cells and septate mycelium which falls apart into arthrospores.
A novel tyrosineinducible tyrosine ammonia lyase enzyme was isolated from the yeast trichosporon cutaneum. Figure 1a shows the impact of yeast extract supplementation on glucose consumption, cell growth and lipid accumulation of t. The genus trichosporon includes 6 species of clinical relevance that may cause superficial infections, such as white piedra and onychomycosis, or deep and invasive infections with high mortality rates. Trichosporon mucoides has been reported from several cases of kidney, heart. In particular, the name trichosporon beigelii is now obsolete, and previously described infections reported in the literature under this name could in fact be due to any one of the species listed below. An trichosporon cutaneum in nahilalakip ha genus nga trichosporon, ngan familia nga trichosporonaceae. Growth of candidafamata and trichosporon cutaneum on uric acid as the sole source of carbon and energy, a hitherto unknown property of yeasts w. It is a yeastlike fungus, often found in soil, decomposing wood, air, rivers, lakes, seawater, cheese, scarab beetles, bird droppings, bats, pigeons and cattle. Disseminated trichosporon beigelii cutaneum infection in an. This enzyme has a higher activity for tyrosine than for phenylalanine and is useful for the production of parahydroxycinnamic acid directly from tyrosine. Successful treatment of trichosporon mucoides infection. Trichosporon cutaneum is a dimorphic fungus initially isolated from a dairy factory and, not. Trichosporon cutaneum, and exceptionally trichosporon asahii and trichosporon loubieri 2, 6. Trichosporon asahii infection in a patient with metastatic prostate cancer as an example of an emerging fungal pathogen kyle magee, mlsascpcm.
Both can be a constituent of the normal microbiota but can also cause both superficial and invasive infections in humans. We investigated the possibility of utilizing unusual carbon sources by three yeast strains. Disseminated trichosporon beigelii cutaneum infection in. Therefore, how to upgrade the final products of lignocellulosebased bioprocess to more valuable ones is becoming a more and more important issue. Biochemical conversion of lignocellulose hydrolysates remains challenging, largely because most microbial processes have markedly reduced efficiency in the presence of both hexoses and pentoses. Voriconazole and posaconazole show excellent in vitro activity against trichosporon. Feb 12, 2018 trichosporon species are fungi that commonly inhabit the soil. Single cell oil sco production from lignocelluloses by oleaginous microorganisms is still high in production cost, making the subsequent production of biofuels inviable economically in such an era of low oil prices. Phenol uptake is not directly linked to its ohydroxylation inside the cell, the first step of phenol metabolism. Invasive trichosporon cutaneum beigelii infection in a patient with relapsed acute myeloid leukemia undergoing bone. Most are typically isolated from soil, but several species occur as a natural part of the skin microbiota of humans and other animals. Rare case of trichosporon mucoides skin infection in an. Fortunately in the recent years, studies of trichosporon epidemiology, virulence factors 15,16, antifungal susceptibility 16,17, and animal models of infection 18,19 have been conducted. They colonize the skin and gastrointestinal tract of humans.
Trichosporon asahii septic thrombophlebitis following. The former species is the imperfect state ofdebaryomyces hansenii zopf. Trichosporon asahii an overview sciencedirect topics. Pdf comparison of growth kinetics and phenol metabolizing. Trichosporon beigelii is a minor component of normal skin flora, and is widely distributed in nature, but nevertheless is the most significant pathogen in the genus trichosporon. Enzyme and microbial technology 34 2004 242247 comparison of growth kinetics and phenol metabolizing enzymes of trichosporon cutaneum r57 and mutants with modified degradation abilities zlatka alexievaa a. Thus, identification of microorganisms capable of efficient and simultaneous utilization of both glucose and xylose is pivotal to improving this process. In this study, we evaluated colony pcr, which directly uses colonies or trace clinical samples as the template for amplification, for rapid. The reevaluation of the genus trichosporon has led to the replacement of the old taxon trichosporon beigelii by six new species.
Trichosporon species and blastoschizomyces capitatus are related fungi and are rare causes of invasive infection in humans. Dimorphism of trichosporon cutaneum and impact on its lipid. Trichosporon is a genus of anamorphic fungi in the family trichosporonaceae. Trichosporon cutaneum definition of trichosporon cutaneum. A 23yearold woman presented with trichosporon cutaneum, which invaded deep into the dermis and subcutaneous tissue of the face and developed chronic. Soilliving yeasts use flavin adenine dinucleotide fadcontaining enzymes to hydroxylate phenols. Trichosporonosis is a potentially lifethreatening disseminated infection with trichosporon beigelii, the causative agent of white piedra.
Identification, genotyping, and pathogenicity of trichosporon spp. Trichosporon asahii infection in a patient with metastatic. The colonies are usually raised and have a waxy appearance, which develop radial furrows and irregular folds. University of groningen growth of candida famata and. Successful treatment of trichosporon mucoides infection with fluconazole in a heart and kidney. Single cell oil production by trichosporon cutaneum from.
Trichosporon asahii is an emerging, lifethreatening opportunistic pathogen. For full access to this pdf, sign in to an existing account, or purchase an annual. Publishers pdf, also known as version of record publication date. An trichosporon cutaneum in uska species han fungi in nahilalakip ha divisio nga basidiomycota, ngan nga syahan ginhulagway ni beurm. Aug 29, 2019 compared to the oleaginous yeast yarrowia lipolytica, trichosporon cutaneum can metabolize pentose sugars more efficiently, and in the meantime is more tolerant to inhibitors, which is suitable for lipid production from lignocellulosic biomass. International journal of analytical chemistry hindawi. We describe the first human infection with a related organism, t. Trichosporon cutaneum an overview sciencedirect topics. Alternatively, you can download the file locally and open with any standalone pdf reader. The phenol hydroxylase from trichosporon cutaneum hydroxylates phenol to catechol. Disseminated trichosporon beigelii cutaneum infection in an artificial. However, case reports of invasive trichosporonosis have been frequently published and the genus is. Invasive infections caused by trichosporon species and. Simultaneous utilization of glucose and xylose for lipid.
Sequencing of the ribosomal dna rdna intergenic spacer 1 igs1 is currently mandatory for accurate trichosporon identification, but it is not usually performed in routine laboratories. Trichosporon asahii, urinary tract infection trichosporon behrend is a genus of anamorphic yeasts yeast can be isolated from the sputum, the urine, the skin and. Trichosporon pullulans phadia setting the standard. Pdf bioremediation of chromium ions with filamentous. This led to the use of two names for this fungus with clinical relevance. However, this species experiences dimorphic transition between yeastform cells and hyphae during submerged fermentation, which consequently affects. The crystal structure model of phenol hydroxylase has been corrected for 11 sequence errors and refined against new data to 1.
Aug 24, 2011 biochemical conversion of lignocellulose hydrolysates remains challenging, largely because most microbial processes have markedly reduced efficiency in the presence of both hexoses and pentoses. The phenol hydroxylase from trichosporon cutaneum hydroxylates phenol to. Comparison of growth kinetics and phenol metabolizing enzymes of trichosporon cutaneum r57 and mutants with modified degradation abilities. Trichosporon cutaneum ifo 0173 is available from the institute for fermentation, osaka. Phenol utilization by filamentous yeast tric hosporon cutaneum. Antonie van leeuwenhoek 49 1983 3668 growth of candidafamata and trichosporon cutaneum on uric acid as the sole source of carbon and energy, a hitherto unknown property of yeasts w. The culture medium consists of 5% glycerol, 5% corn steep liquor, 1% mineral mixture and 1% 1 m. The ability to adhere to and form biofilms on implanted devices can account for the progress of invasive trichosporonosis, as it can promote the escape from antifungal drugs and host immune responses. Trichosporon cutaneum r57 and it s derivative strain nz94 are able to accumulale significant cellular amounts of methionine.
The name trichosporon cutaneum is often used synonymously with trichosporon beigelii. Urinary tract infection by trichosporon asahii s sood, d pathak, r sharma, s rishi abstract trichosporon asahii is a basidiomycetous yeast which causes white piedra and onychomycosis in immunocompetent hosts as well as various localized and disseminated invasive infections in immunodeficient hosts. The soil yeast trichosporon cutaneum, which is distinguished by having a strictly oxidative metabolism, can be induced to utilize phenol as a sole carbon source. Systemic infection by this fungus has been most frequently described in immunocompromised hosts with neutropenia, but one case has been reported in the acquired immunodeficiency syndrome setting. Yeast growth and lipid synthesis are regulated by nutritional conditions, particularly nitrogen limitation, through which amino acid metabolism is regulated to redirect carbon flux to lipid synthesis. This superficial mycosis occurs mostly in temperate and tropical regions and is rarely seen in north. From the eye hospital, khartoum, and the microbiology mycology department, faculty. Trichosporon beigelii, adopted by physicians, and trichosporon. Bioremediation of chromium ions with filamentous yeast trichosporon cutaneum r57. He had had an aortic valve replacement for rheumatic heart disease 3 years earlier. The higher resolution data together with careful exploitation of noncrystallographic symmetry restraints and the use of many small groups for refinement of anisotropic displacement parameters resulted in a large decrease in the crystallographic r factor. Comparative analysis of the function and regulation of aspartate kinase and homoserine dehydrogenase in both strains was carried out. For many years, the unique species of the genus, trichosporon beigelli, was only known as an environmental and saprophytic fungus occasionally found as the etiological agent of white piedra. Five species of trichosporon delineated by these new criteria have been associated with humans.
Six of them are pathogenic to humans and occupy different niches on the. Transport and hydrolysis of disaccharides by trichosporon. All species of trichosporon are yeasts with no known teleomorphs sexual states. Growth of candidafamata and trichosporon cutaneum on uric acid as the sole source of carbon and energy, a hitherto unknown property of yeasts. Invasive infections with trichosporon beigelii also called cutaneum or t.
Aug 01, 2015 arthrospores are a primitive spore type formed by the fragmentation of the hyphal strand through the septation point. Bloodstream infections due to trichosporon spp species. Trichosporon cutaneum has been reported to be the most common yeast in onychomycosis 5,7,8. Trichosporon asahii is the major pathogen causing invasive trichosporonosis. However, case reports of invasive trichosporonosis have been frequently published and the. Us6951751b2 dna and amino acid sequences of a tyrosine. However, this fungus has been recognized as an opportunistic agent of invasive infections, mostly in cancer patients and those exposed to invasive medical procedures. Trichosporon sepsis and leukemia trichosporon sepsis and leukemia rivera, robert. Facial granuloma associated with trichosporon cutaneum infection. Proliferation of trichosporon yeasts in the hair can lead to an unpleasant but nonserious condition.
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